Creating a Cellular Component (CC) only annotation

• Click on the arrow to the left of the ‘Create Activity’ icon.
• Select ‘Create New CC Annotation’.
• Using C. elegans as an example:
  • Type in gene name: skn-1
  • Select the WBGene entry from the dropdown list
  • Gene products link out to respective gene product pages via dbxrefs.yaml

• Note that the CC only form creates annotations stating only that a gene product is ‘located in’ a given GO CC and is not making any claims about where the MF of that gene product may occur.

• Add the CC
  • Search for ‘nucleus’
  • Autocomplete options are presented in a pop-up window
  • Terms link out to AmiGO

  • Evidence code autocomplete using ECO term names

    • Search for ‘direct assay’
    • GO codes are indicated with bold three-letter GO code
  • Add a reference. Two options:
    • Cut and paste a DB and id, e.g. PMID:30442878 OR

    • Click on the +/lines symbol

      • Pop-up appears with a select drop-down of dbs for references: PMID, DOI, GO_REF
      • Select PMID
      • Add identifier: 30442878
• Adding contextual information to a CC only annotation can be done as for Activity Units.